Deep Learning based Digital Cell Profiles for Risk Stratification of Urine Cytology Images

Deep Learning based Digital Cell Profiles for Risk Stratification of Urine Cytology Images

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Urine cytology is a take a look at for the detection of high-grade bladder most cancers. In medical follow, the pathologist would manually scan the pattern beneath the microscope to find atypical and malignant cells. They would assess the morphology of these cells to make a analysis. Accurate identification of atypical and malignant cells in urine cytology is a difficult process and is a vital half of figuring out totally different analysis with low-risk and high-risk malignancy.
Computer-assisted identification of malignancy in urine cytology might be complementary to the clinicians for remedy administration and in offering recommendation for finishing up additional exams. In this research, we introduced a technique for figuring out atypical and malignant cells adopted by their profiling to foretell the chance of analysis mechanically.
For cell detection and classification, we employed two totally different deep learning-based approaches. Based on finest performing community predictions on the cell-level, we recognized low-risk and high-risk instances utilizing the depend of atypical cells and the overall depend of atypical and malignant cells. The space beneath the ROC curve reveals {that a} whole depend of atypical and malignant cells is comparably higher at analysis as in comparison with the depend of malignant cells solely.
We obtained space beneath the ROC curve with the depend of malignant cells and the overall depend of atypical and malignant cells as 0.81 and 0.83 respectively. Our experiments additionally reveal that the digital threat could possibly be a greater predictor of the ultimate histopathology based analysis. We additionally analyzed the variability in annotations at each cell and entire slide picture degree and in addition explored the attainable inherent rationales behind this variability. This article is protected by copyright. All rights reserved.

COMPARISON OF FECAL CYTOLOGY AND PRESENCE OF CLOSTRIDIUM PERFRINGENS ENTEROTOXIN IN CAPTIVE BLACK-FOOTED FERRETS ( MUSTELA NIGRIPES) BASED ON DIET AND FECAL QUALITY

The black-footed ferret (Mustela nigripes) is an endangered mustelid native to North America. Gastroenteritis is a documented trigger of morbidity and mortality in managed people, notably by infectious brokers. Fecal cytology is a cheap and speedy take a look at that may assist information medical administration methods for animals with enteritis; nonetheless, regular parameters haven’t been established in black-footed ferrets. The goal of this research was to characterize fecal cytological findings of 50 fecal samples from 18 black-footed ferrets that obtained two totally different weight-reduction plan sorts (floor meat versus entire prey) and that had been visibly judged to be regular or irregular.
This research additionally examined for the presence of Clostridium perfringens enterotoxin by enzyme-linked immunosorbent assay in all irregular and a subset of regular fecal samples. Significantly increased spore-forming micro organism and yeast prevalence had been current in regular feces from people following the meat-based in contrast with the whole-prey weight-reduction plan.
Samples from people with irregular feces had considerably extra spore-forming micro organism than regular feces, regardless of weight-reduction plan. Normal feces had increased diplococci and spore-forming micro organism in contrast with home canine and feline requirements. A single irregular fecal pattern was optimistic for enterotoxin and originated from the one animal requiring remedy.
Results point out that low numbers of spore-forming micro organism might be present in fecal samples from clinically regular black-footed ferrets. Fecal cytology reveals considerably elevated spore-formers in clinically irregular ferrets and in clinically regular ferrets following a floor meat-based weight-reduction plan.

The prognostic affect of peritoneal washing cytology for in any other case resectable extrahepatic cholangiocarcinoma sufferers

The medical significance of peritoneal washing cytology (PWC) for cholangiocarcinoma sufferers stays unclear. The medical knowledge of 137 extrahepatic cholangiocarcinoma sufferers who obtained PWC and healing surgical procedure had been retrospectively analyzed. Among the 137 sufferers analyzed, 5 (3.6%) had optimistic PWC, and 132 (96.4%) had unfavorable PWC.
The median survival time in sufferers with unfavorable PWC was 6.45 years, and the general 1-, 2-, and 5-year survival charges had been 86.5%, 75.3%, and 51.6%, respectively. The median survival time in sufferers with optimistic PWC was 2.56 years, and the general 1-, 2-, and 5-year survival charges had been 60.0%, 60.0%, and 40.0%, respectively.
Deep Learning based Digital Cell Profiles for Risk Stratification of Urine Cytology Images
A multivariate evaluation revealed that optimistic lymph node metastasis (P < 0.001), optimistic perineural invasion (P = 0.014) and no use of adjuvant chemotherapy (P < 0.001), however not optimistic PWC had been independently related to a worse total survival. In conclusion, surgical procedure and subsequent chemotherapy may be a therapeutic choice for cholangiocarcinoma sufferers with optimistic PWC.

Diagnostic mesothelioma biomarkers in effusion cytology

Malignant mesothelioma is a uncommon malignancy with a poor prognosis whose growth is said to asbestos fiber publicity. An growing position of genetic predisposition has been acknowledged not too long ago. Pleural biopsy is the gold commonplace for analysis, during which the identification of pleural invasion by atypical mesothelial cell is a significant criterion.
Pleural effusion is often the primary signal of illness; subsequently, a cytological specimen is usually the preliminary or the one specimen obtainable for analysis. Given that reactive mesothelial cells might present marked atypia, the analysis of mesothelioma on cytomorphology alone is difficult. Accordingly, cell block preparation is inspired, because it permits immunohistochemical staining.
Traditional markers of mesothelioma equivalent to glucose transporter 1 (GLUT1) and insulin-like progress issue 2 mRNA-binding protein 3 (IMP3) are informative, however tough to interpret when reactive proliferations aberrantly stain optimistic. BRCA1-associated protein 1 (BAP1) nuclear staining loss is very particular for mesothelioma, however sensitivity is low in sarcomatoid tumors.
Cyclin-dependent kinase inhibitor 2A (CDKN2A)/p16 homozygous deletion, assessed by fluorescence in situ hybridization, is extra particular for mesothelioma with higher sensitivity, even within the sarcomatoid variant. The surrogate marker methylthioadenosine phosphorylase (MTAP) has been discovered to reveal wonderful diagnostic correlation with p16.

AnaPrep Blood DNA Extraction Kit
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K5019100 1 kit
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D100T 1 kit (50 tubes)
EUR 469
Description: DNA Extraction Kit in Tubes by Cygnus Technologies is available in Europe via Gentaur.

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SD25 25 preps
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Z1322002 1 kit (48 extractions) Including all required plastic disposables
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DC301-01 100 rxn
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23793 50 preps
EUR 75

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FAPDE-100 100 preps
EUR 129

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EUR 274

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FAPDE-001 100 preps
EUR 140

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FAPDE-001-1 300 preps
EUR 201

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FAPDE-300 300 preps
EUR 172

Food DNA Extraction Kit I(50prep)
FDK-1050 50 preps
EUR 274

Rapid Animal Genomic DNA Extraction Kit
AT4780 10Preps
EUR 69.03

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AT4781 50Preps
EUR 126.13

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AT4782 250preps
EUR 354.5

Rapid Yeast Genomic DNA Extraction Kit
BS8227 50Preps
EUR 76.64

One-Tube Tissue DNA Extraction Kit
BS8401 100preps
EUR 78.93

One-Tube Hair DNA Extraction Kit
BS8407 50Preps
EUR 78.93

Rapid Blood Genomic DNA Extraction Kit
BT4782 50Preps
EUR 76.64

One-Tube Swab DNA Extraction Kit
T71612 20Preps
EUR 66.75

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T71613 100preps
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T71614 500Preps, 500prep
EUR 247.93

HiYieldGel/PCR DNA Fragments Extraction Kit
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31030-250 1kit
EUR 545
Description: Minimum order quantity: 1 unit of 1kit

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31030-50 1kit
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Description: Minimum order quantity: 1 unit of 1kit

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D105 30 ml
EUR 204
Description: DNA Extraction Buffer for wells by Cygnus Technologies is available in Europe via Gentaur.

DNA Extraction Buffer for tubes
D108 30 ml
EUR 204
Description: DNA Extraction Buffer for tubes by Cygnus Technologies is available in Europe via Gentaur.

Plant Genomic DNA extraction buffer
20-abx098075
  • EUR 286.00
  • EUR 105.00
  • 100 ml
  • 1.2 ml

Genomic DNA Extraction and Purification
EP10012 100 Tests
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FFPE Tissue DNA Extraction Kit - Magnetic Beads
K5011450 1 kit
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Agarose gel DNA extraction and purification kit
K1441-100
EUR 425

Blood genomic DNA extraction and purification kit
K1443-100
EUR 479

Blood Genomic DNA Extraction Mini Kit (100prep)
FABGK-100 100 preps
EUR 165

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FABGK-300 300 preps
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FAPDE-300-CAPLESS 300 preps
EUR 172

Plant Genomic DNA Extraction Mini Kit (50prep)
FAPGK-001 50 preps
EUR 158

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FAPGK-001-2 200 preps
EUR 274

Plant Genomic DNA Extraction Maxi Kit (10prep)
FAPGK-002 10 preps
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FAPGK-002-1 24 preps
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Forensic DNA Extraction Kit (Proteinase K incl.)
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FD25 25 preps
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FE25 25 preps
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Bacterial DNA Extraction Kit (Proteinase K included)
BA05 5 preps Ask for price

Bacterial DNA Extraction Kit (Proteinase K included)
BA100 100 preps
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BA50 50 preps
EUR 100

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One-Tube Clinical Sample DNA Extraction Kit
BS8403 100preps
EUR 78.93

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BS8404 500Preps, 500prep
EUR 165.71

Plasmid DNA Extraction Kit (Rnase A included)
PL05 5 preps Ask for price

Plasmid DNA Extraction Kit (Rnase A included)
PL100 100 preps
EUR 130

Plasmid DNA Extraction Kit (Rnase A included)
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PL50 50 preps
EUR 94

Plant DNA Extraction Kit (Proteinase K included)
PT05 5 preps Ask for price

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PT100 100 preps
EUR 138

Plant DNA Extraction Kit (Proteinase K included)
PT50 50 preps
EUR 100

One-tube Plant Genomic DNA Extraction Kit
PT71816 100preps
EUR 88.06

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PT71817 500Preps, 500prep
EUR 202.25

Tissue DNA Extraction Kit (Proteinase K included)
TD05 5 preps Ask for price

Tissue DNA Extraction Kit (Proteinase K included)
TD100 100 preps
EUR 138

Tissue DNA Extraction Kit (Proteinase K included)
TD50 50 preps
EUR 100

Histone/DNA Binding Protein Extraction Kit (cells)
P514-25 - Ask for price

Histone/DNA Binding Protein Extraction Kit (cells)
P514-4 - Ask for price

Histone/DNA Binding Protein Extraction Kit (cells)
P514-50 - Ask for price

Mouse Tail DNA Extraction Kit (250 reactions)
T605 - Ask for price

Mouse Tail Genomic DNA Extraction Kit(250)
M9100-250 250 prep
EUR 536

Agarose Gel DNA Extraction and Purification
EP10010 100 Tests
EUR 297

Genomic DNA Extraction Mini KitPlant Tissue
FYG103-100P 100 Preps Ask for price

DNA Extraction Control 670, (Red Cap)
BIO-35028 500 rxns Ask for price

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Human Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2H 1 Kit
EUR 430

Mouse Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2M 1 Kit
EUR 430

Rat Real-time PCR DNA Quantitation after QPCR DNA Damage Analysis Kit
DD2R 1 Kit
EUR 430

Human Mitochondrial DNA Copy Number Kit
MCN1 1 Kit
EUR 349

Rat Mitochondrial DNA Copy Number Kit
MCN2 1 Kit
EUR 349

Mouse Mitochondrial DNA Copy Number Kit
MCN3 1 Kit
EUR 349

LumiPure genomic DNA from AnySample Kit, 50
21573 50 preps
EUR 108

cfPure® MAX Cell-Free DNA Extraction Kit
K5011625MA 1 kit
EUR 498

Cell & tissue genomic DNA extraction and purification kit
K1442-100
EUR 566

96-well Genomic DNA Extraction Kit (1 plates)
FADWE-96001 1 prep
EUR 158

96-well Genomic DNA Extraction Kit (2 plates)
FADWE-96002 2 preps
EUR 213

96-well Genomic DNA Extraction Kit (4 plates)
FADWE-96004 4 preps
EUR 317

Fungi/ Yeast Genomic DNA Extraction Mini Kit(50prep)
FAFYG001 50 preps
EUR 274

Fungi/ Yeast Genomic DNA Extraction Mini Kit(100prep)
FAFYG001-1 100 preps
EUR 390

Plasmid DNA Extraction Midi Kit (25prep), Ion Exchange
FAPDE-002 25 preps
EUR 216

Plasmid DNA Extraction Midi Kit (50prep), Ion Exchange
FAPDE-002-1 50 preps
EUR 317

Plasmid DNA Extraction Maxi Kit (10prep), Ion Exchange
FAPDE-003 10 preps
EUR 201

Plasmid DNA Extraction Maxi Kit (20prep), Ion Exchange
FAPDE-003-1 20 preps
EUR 281

Plasmid DNA Extraction Mini Kit (Automatic Machine) Sample
FAPDE-AUTO-024 24 preps
EUR 140

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001 1 prep
EUR 146

96-well Plasmid DNA Extraction Kit (2 plates)
FAPWE-96002 2 preps
EUR 188

EZ-10 Spin Column DNA Gel Extraction Kit
BS353 50Preps
EUR 76.64

EZ-10 Spin Column DNA Gel Extraction Kit
BS354 100preps
EUR 95.68

EZ-10 Spin Column DNA Gel Extraction Kit
BS654 250preps
EUR 166.58

FitAmp Blood and Cultured Cell DNA Extraction Kit
P-1018 100 Samples
EUR 390.75
Description: fast delivery possible

Miniprep Kit For Plasmid DNA Extraction And Purification
MB1005-100Reactions 100 Reactions
EUR 143

Miniprep Kit For Plasmid DNA Extraction And Purification
MB1005-250Reactions 250 Reactions
EUR 259

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EUR 120

Glycerol Assay Kit (For liquid samples)
abx090674-1Kit 1 Kit
EUR 613

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Genomic DNA from Lupus: Colon, from a single donor
D1236090Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from hypertension: Heart, from a single donor
D1236122Hd-2 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Heart, from a single donor
D1236122Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from hypertension: Kidney, from a single donor
D1236142Hd-2 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Kidney, from a single donor
D1236142Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Liver, from a single donor
D1236149Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Asthma: Lung, from a single donor
D1236152Ld-1 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Bronchitis: Lung, from a single donor
D1236152Ld-2 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Emphysema: Lung, from a single donor
D1236152Ld-3 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Pneumonia: Lung, from a single donor
D1236152Ld-4 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Lung, from a single donor
D1236152Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Pancreas, from a single donor
D1236188Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Spleen, from a single donor
D1236246Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Stomach, from a single donor
D1236248Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Lupus: Uterus, from a single donor
D1236274Lup 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Arteriosclerosis: Aorta, from a single donor
D1236012Hd-4 50 ug
EUR 984
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from hypertension: Artery, from a single donor
D1236013Hd-2 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from hypertension: Vein, from a single donor
D1236020Hd-2 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

DNA-PK Colorimetric Cell-Based ELISA Kit
EKC1173 100ul
EUR 572

cfPure® Cell Free DNA Extraction Kit, 100 ml
K5011610 1 kit
EUR 448

cfPure® Cell Free DNA Extraction Kit, 250 ml
K5011625 1 kit
EUR 748

cfPure® MAX V2 Cell-Free DNA Extraction Kit
K5011625MA-V2 1 kit
EUR 498

Blood Genomic DNA Extraction MIni Kit (Automatic Machine) Sample
FABGK-AUTO-024 24 preps
EUR 150

Plasmid DNA Extraction Maxi Kit (4prep), FavorFilter, Ion Exchange
FAFTE-001 4 preps
EUR 150

Plasmid DNA Extraction Maxi Kit (10prep), FavorFilter, Ion Exchange
FAFTE-001-1 10 preps
EUR 208

Plasmid DNA Extraction Midi Kit (25prep), FavorFilter, Ion Exchange
FAFTE-002 25 preps
EUR 245

Plasmid DNA Extraction Midi Kit (50prep), FavorFilter, Ion Exchange
FAFTE-002-1 50 preps
EUR 368

Plasmid DNA Extraction Midi Kit (25prep), EndoToxinFree, Ion Exchange
FAPDE-002-EF 25 preps
EUR 230

96-well Plant Genomic DNA Extraction Kit (1 plates)
FAPGE-96001 1 prep
EUR 194

96-well Plant Genomic DNA Extraction Kit (2 plates)
FAPGE-96002 2 preps
EUR 281

96-well Plant Genomic DNA Extraction Kit (4 plates)
FAPGE-96004 4 preps
EUR 448

96-well Viral RNA/DNA Extraction Kit (1 plates)
FAVRE-96001 1 prep
EUR 194

96-well Viral RNA/DNA Extraction Kit (2 plates)
FAVRE-96002 2 preps
EUR 281

96-well Viral RNA/DNA Extraction Kit (4 plates)
FAVRE-96004 4 preps
EUR 448

Tissue / Blood COMBI DNA Extraction Kit (Proteinase K incl.)
BT05 5 preps Ask for price

Tissue / Blood COMBI DNA Extraction Kit (Proteinase K incl.)
BT50 50 preps
EUR 120

Genomic DNA from Mouse Normal Tissue: Brain
D1334035 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Heart
D1334122 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Kidney
D1334142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Liver
D1334149 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Lung
D1334152 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Spleen
D1334246 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Mouse Normal Tissue: Stomach
D1334248 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Brain
D1434035 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Heart
D1434122 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Kidney
D1434142 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Liver
D1434149 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Lung
D1434152 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Rat Normal Tissue: Stomach
D1434248 100 ug
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
The goal of this evaluate is to supply a vital appraisal of the literature concerning the diagnostic worth of many of these rising biomarkers for malignant mesothelioma in effusion cytology.