Biomarkers predicting oncological outcomes of high-risk non-muscle-invasive bladder cancer.

The European Organization for Research and Treatment of Cancer (EORTC) and the Spanish Urological Club for Oncological Treatment (CUETO) scoring systems show limited accuracy for the prediction of disease recurrence and progression of non-muscle-invasive bladder cancer (NMIBC).

This aspect is even more relevant in the category of HR NMIBC. Biomarkers might potentially help to further categorize the outcomes of these patients. Therefore, we sought to review the evidence available on tissue-based, urinary, and serum biomarkers for the prediction of recurrence, progression, and survival in HR NMIBC.A systematic literature review without time restrictions was performed using PubMed/EMBASE, Web of Science, SCOPUS, and the Cochrane Libraries.

The search was filtered for articles in the English, Italian, German, French, and Spanish languages, involving patients with more than 18 years of age. Relevant papers on tissue-based, serum and urinary biomarkers related to the prediction of oncological outcomes for high-risk bladder cancer patients were included in the analyses.Overall, 71 studies were eligible for inclusion in this review.

The majority of the investigations performed so far focused on immunohistochemical analyses on tumoral tissue. Overall, p53 was the most studied biomarker, but results regarding its prognostic and predictive role were contradictory. Ki67 seems to be a promising biomarker in the prediction of recurrence.

Recently, PD-L1 has been associated with the prediction of recurrence free survival and of treatment-refractory disease. Markers developed un urine samples are focused on commercially available kits, which currently do not unequivocally show strongly superior levels of accuracy to cytology.

However, they have demonstrated to be potentially helpful in the prediction of recurrence. Blood-based biomarkers represent an emerging reality with promising future applications.

AIM-100

A3148-100 100 mg
EUR 1859
Description: AIM-100 is a small inhibitor of Ack1 tyrosine kinase with IC50 value of 24 nM [1]. AIM-100 mimicked ATP and inhibited the activity of Ack1 significantly and specifically.

MTBK_20640-100

AR02-P0011-100 100ug
EUR 1133

hCRP FRET 100

CH1008-100 1 Kit
EUR 212

hCRP TRF 100

CHTR1008-100 1 Kit
EUR 239

hIgG TRF 100

HITR1011-100 1 Kit
EUR 239

hInsulin FRET 100

IH1010-100 1 Kit
EUR 212

hInsulin TRF 100

IHTR1010-100 1 Kit
EUR 239

hAlbumin FRET 100

AH1005-100 1 Kit
EUR 212

hAlbumin TRF 100

AHTR1005-100 1 Kit
EUR 239

rIgG FRET 100

RI1015-100 1 Kit
EUR 212

S-100 Antibody

3958-100
EUR 316

Etoposide (100 mM)

1043-100
EUR 153

100 BlotBot Reagent Reservoirs

RRVR-100 100pack
EUR 177
Description: BlotBot reagent reservoirs will allow dispensing of unique reagents during processing. Pack of 100.

GFP Antibody, 100 uL

P601-100 - Ask for price

CD1b(100-1A5) Antibody

BNC041044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF405S conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC471044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF647 conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNCAP1044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNCB1044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), Biotin conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC811044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF680R conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC701044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF770 conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC881044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF488A conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC611044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF660R conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC681044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF568 conjugate, Concentration: 0.1mg/mL

FUNNEL, 100 MM, PP

6120P-100 12/pk
EUR 47
Description: Reusable Plastics; Reusable Funnels

CD1b(100-1A5) Antibody

BNC051044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF405M conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNUB1044-100 100uL
EUR 209
Description: Primary antibody against CD1b(100-1A5), Concentration: 0.2mg/mL

CD1b(100-1A5) Antibody

BNC431044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF543 conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNCH1044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC551044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF555 conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC941044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF594 conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC801044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF680 conjugate, Concentration: 0.1mg/mL

CD1b(100-1A5) Antibody

BNC401044-100 100uL
EUR 199
Description: Primary antibody against CD1b(100-1A5), CF640R conjugate, Concentration: 0.1mg/mL

Mouse IgM FRET 100

IM1009-100 1 Kit
EUR 212

Mouse IgG FRET 100

MIG1012-100 1 Kit
EUR 212

ATP Solution (100 mM)

2121-100
EUR 126

PBS Tablets (100 ml)

2865-100
EUR 164

Rv0351 (GrpE)-100

AR02-P0003-100 100ug
EUR 266

Rv1009 (RpfB)-100

AR02-P0006-100 100ug
EUR 1133

Rv2450c (RpfE)-100

AR02-P0007-100 100ug
EUR 1133

Rv3875 (esxA)-100

AR02-P0008-100 100ug
EUR 1133

Rv2031c(HspX)-100

AR02-P0009-100 100ug
EUR 1133

Rv3131 (NfnB)-100

AR02-P0010-100 100ug
EUR 1133

Biotin TRF 100

BTTR-1006-100 1 Kit
EUR 239

100 bp DNA Ladder (100 bp ~ 1,500 bp, with loading dye)

W0636-100 - Ask for price

Reflex Clips, 7mm , 100/Pk

LC1001-100 100/pk
EUR 147

ERLENMEYER FLASK, 100 ML, PP

4985P-100 6/pk
EUR 54
Description: Reusable Plastics; Reusable Erl Flasks

ERLENMEYER FLASK, 100 ML, PMP

4990P-100 6/pk
EUR 57
Description: Reusable Plastics; Reusable Erl Flasks

bcl-2(100/D5) Antibody

BNC040045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF405S conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC470045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF647 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC550045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF555 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNCB0045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), Biotin conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC810045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF680R conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC700045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF770 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC880045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF488A conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC940045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF594 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC680045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF568 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNCAP0045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC050045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF405M conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC400045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF640R conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNUB0045-100 100uL
EUR 209
Description: Primary antibody against bcl-2(100/D5), Concentration: 0.2mg/mL

bcl-2(100/D5) Antibody

BNC430045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF543 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNCH0045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC610045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF660R conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC800045-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5), CF680 conjugate, Concentration: 0.1mg/mL

Bridge-It cAMP-PDE 100

PD1007-100 1 Kit
EUR 239

Triton® X-100 Detergent

B2394-100
EUR 109

S-100?? Protein, Bovine Brain

P1275-100
EUR 403

2.5x12 cellogel 250µ (100)

EHCA1200-ST01-100 ea
EUR 87

2.5x17 cellogel 200µ (100)

EHCA1200-ST12-100 ea
EUR 123

4x17 cellogel 250µ (100)

EHCA1200-ST21-100 ea
EUR 169

4x17 cellogel 200µ (100)

EHCA1200-ST22-100 ea
EUR 169

5.7x14 cellogel 250µ (100)

EHCA1200-ST36-100 ea
EUR 160

5.7x14 cellogel 200µ (100)

EHCA1200-ST37-100 ea
EUR 160

5.7x17 cellogel 250µ (100)

EHCA1200-ST77-100 ea
EUR 223

Rv2769c (PE27 protein)-100

AR02-P0001-100 100ug
EUR 479

Microscope Slide Boxes,100-Place, Blue

LC7002-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Green

LC7003-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Red

LC7004-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, Yellow

LC7005-100 100/CS
EUR 806

Microscope Slide Boxes,100-Place, White

LC7006-100 100/CS
EUR 806

bcl-2(100/D5 + 124) Antibody

BNC041234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF405S conjugate, Concentration: 0.1mg/mL

Sodium Pyruvate Solution, 100 mM Solution

CCM1753-100 100 mL
EUR 60.84

bcl-2(100/D5 + 124) Antibody

BNC471234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF647 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNCAP1234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC801234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF680 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC811234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF680R conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC681234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF568 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC701234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF770 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC881234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF488A conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC611234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF660R conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNUB1234-100 100uL
EUR 209
Description: Primary antibody against bcl-2(100/D5 + 124), Concentration: 0.2mg/mL

bcl-2(100/D5 + 124) Antibody

BNCH1234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC051234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF405M conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC431234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF543 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNCB1234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), Biotin conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5 + 124) Antibody

BNC551234-100 100uL
EUR 199
Description: Primary antibody against bcl-2(100/D5 + 124), CF555 conjugate, Concentration: 0.1mg/mL

Despite a long history of attempts to discover accurate biomarkers predicting oncological outcomes for HR NMIBC, contradictory or uncertain findings render the adoption of this ancillary techniques in clinical practice still unlikely. Future attempts should be directed to the development of prospective trials and the definition of standardized cut-off levels to render findings worthy of comparison.